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GFP staining with FACS

Started by ananas, Jul 19 2013 08:49 AM

DC GFP EGFP

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1 reply to this topic

#1 ananas

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Posted 19 July 2013 - 08:49 AM

Hi,
i am working on a very rare subset of mouse DC. Now I got an EGFP-mouse for my protein of interest, but could not detect GFP pos. cells in mouse DC of skindraining LN. The protein is probably intracellularly, but lysis did not help. I only expect very low numbers of
cells - but compared to my non-EGFP controll cells, I could not detect any...

Does anyone have experience with using an anti-GFP antibody (which clone?) in FACS to enhance the signal? Does that make sense? I assumed the GFP signal to be strong enough - if present. I am wrong?

thanks a lot!

#2 Curtis

Metaller Scientist

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Excellent

Posted 20 July 2013 - 02:58 AM

Anti-gfp ab290 raised in rabbit is great.

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