Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

DNA in 1water:0,1TE at 37 degrees


  • Please log in to reply
1 reply to this topic

#1 Lfs

Lfs

    member

  • Active Members
  • Pip
  • 25 posts
1
Neutral

Posted 19 July 2013 - 03:59 AM

Hi guys!

I forgot my DNA in 1:0,1 TE at 37 degrees for more than 24 hours!

Yesterday I was doing the isopropanol precipitation, and after the first ethanol wash I should incubate my DNA for 1 hour at 37*C...Well, I only remembered about it when I was in my bed Posted Image .

Did I mess up?

Thanks!

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,758 posts
130
Excellent

Posted 19 July 2013 - 04:39 AM

not necessarily. dna is a pretty stable at pH above 7. the only concern is if something (fungus?) is growing in the solution.
talent does what it can
genius does what it must
i do what i get paid to do




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.