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DNA in 1water:0,1TE at 37 degrees

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#1 Lfs



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Posted 19 July 2013 - 03:59 AM

Hi guys!

I forgot my DNA in 1:0,1 TE at 37 degrees for more than 24 hours!

Yesterday I was doing the isopropanol precipitation, and after the first ethanol wash I should incubate my DNA for 1 hour at 37*C...Well, I only remembered about it when I was in my bed Posted Image .

Did I mess up?


#2 mdfenko


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Posted 19 July 2013 - 04:39 AM

not necessarily. dna is a pretty stable at pH above 7. the only concern is if something (fungus?) is growing in the solution.
talent does what it can
genius does what it must
i do what i get paid to do

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