I always done the western blot without any problem but recently, I have a prblem, the LMW don't transfer of gel into membrane correctly while all situations are equal in compare to past ( 0.2 micron membrane, low voltage and time related to transfer etc.) I used nitrocellulose membrane, Other proteins (for example upper 40 kd are transferred heterogenous while homogenous in past ) I mean of hetero-homo related to use ponceau S staining. Can you help me? only element changed is PAG! Do you think change polyacrylamide gel can help me?
Edited by Meysam_Kashiri, 19 July 2013 - 03:17 AM.