co-IP stained with coomassie blue
Posted 19 July 2013 - 12:52 AM
i tried to perform co-IP experiment using protein A beads and tried to stain my gel with coomassie blue just to check.
however i saw only one very strong band corresponding to approximately 55 kda. since my target protein also corresponds to around 50 Kda, i am confused whether the strong band i saw is IgG or my target protein.
there are no other bands i saw from my gel. except for that strong band at 55kDa. what could it possibly be?
Posted 19 July 2013 - 06:32 PM
Posted 26 August 2013 - 08:36 AM
you could include a no antibody control to the experiment to confirm that.