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Why different elution buffers for Protein G and Protein A chromatography?

protein purification affinity chromatography ProteinG Antibody

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#1 AnetteM



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Posted 18 July 2013 - 11:25 PM

I have come across several protocols for affinity purification of antibodies with Protein G and Protein A. In most of them 0.1M Glycin pH 3 is recommended as elution buffer for Potein G while 0.1M Citric acid buffer pH 3 is recommended for Protein A. Can anyone explain to me why this is?

-text edited by Curtis

#2 Curtis


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Posted 20 July 2013 - 06:27 AM

I have seen protocols for protein G with citric acid too, but i normally just add sds-page loading dye since i do wb.

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