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nuclear protein detection in whole-tissue (skin) lysate

XPA whole tissue nuclear lysate western blot

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#1 uciresearch

uciresearch

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Posted 18 July 2013 - 03:47 PM

xpa blots.png
Hi,
I am trying to detect the levels of a nuclear DNA repair protein (named XPA) in mouse whole tissue lysates. I have successfully detected it in mouse brain and liver samples, but not in mouse shaved back whole-skin samples. All samples were collected at the same time/with the same method. The method used for sample prep was that of Sancar et al. 2011 (http://www.ncbi.nlm....les/PMC3082913/). In this paper they show that XPA is indeed expressed in mouse back skin. Could there be any additional important step that they may have performed but not listed on the paper methods?

My only thoughts are that there may be a different form of XPA found in the skin versus brain and liver. We have ordered a different antibody to check (since all they said in Sancar et al was that their Ab was from Santa cruz biotech).


Thank you for your help!





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