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Why do people typically use AP instead of HRP for Southerns?

alkaline phosphatase horseradish peroxidase southern blot

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#1 assembler01

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Posted 18 July 2013 - 08:42 AM

It looks like people usually use AP antibodies for Southern blot detection instead of HRP. We have reagents for doing HRP detection on our western blots though. Wouldn't it be more efficient to stick to one system in the lab? Is there a good reason people mainly use AP for Southern's instead of HRP?

Thanks.

#2 mdfenko

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Posted 18 July 2013 - 10:33 AM

in general, ap is more sensitive than hrp.
talent does what it can
genius does what it must
i do what i get paid to do

#3 bob1

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Posted 18 July 2013 - 12:44 PM

Signal from AP also tails off less rapidly, so you can do longer exposures. HRP is quick and quite bright, but the signal doesn't last.





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