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Mammalian cell culture and G418 selection

G418

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#1 watsonkatherine

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Posted 16 July 2013 - 01:32 PM

I am working with guinea pig fibroblasts, epithelial, and endothelial cells using 400-600ug/mL G418 for selecting cells with my GOI (GFP or mCherry tagged).

I had a general question regarding the neo-resistant gene - our transfected mammalian cells that took our GOI have the neo-resistant gene, and we see some dying off and some surviving during the selection process. However we also see mammalian cells dying / surviving as well. If the mammalian cells do not have the neo-resistant gene how are they surviving>? Did they take up the plasmid and express the SV40 but not the CMV promoter?

#2 Curtis

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Posted 16 July 2013 - 09:39 PM

You asked the same question yesterday and the user leelee replied to it. Did you read her reply?





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