Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

RBC lysis buffer for Rat Blood

RBC lysis rats

  • Please log in to reply
1 reply to this topic

#1 Clare

Clare

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 192 posts
1
Neutral

Posted 16 July 2013 - 02:27 AM

Hi everyone,

Long time no see!

Can anyone recommend a good RBC lysis buffer for rat blood?

I have tried the Biolegend buffer (ammonium chloride, potassium carbonate, and EDTA) and it just doesn't work well compared to when I use mouse blood (I have tried different times etc).

I also tried the DAKO Utilyse kit (which is FANTASTIC for mouse blood) but it didn't work at all for my rat blood.

Any suggestions would be greatly appreciated Posted Image

Clare

#2 xiaogu985

xiaogu985

    member

  • Members
  • Pip
  • 1 posts
1
Neutral

Posted 17 July 2013 - 06:03 AM

Maybe you can try to use ddH2O for 20-30s





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.