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co-ip of endogenous and tagged proteins

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#1 soymilk14



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Posted 15 July 2013 - 04:37 PM

hello everyone,

sorry for my simple basic question, but for studies and experiments, will it be enough to perform co-ip from endogenous proteins? why is it sometimes necessary to transfect his or gst-fused protein and then perform co-IP? is it only for validation? thank you...

#2 Tom M

Tom M


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Posted 16 July 2013 - 01:19 PM

Generally speaking, antibodies for the tag (ie 6His or FLAG or GFP) are more specific than the antibody against the targeted endogenous protein. Therefore, you should have less non-specific interactions with the antibody and then the IP should be cleaner.

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