Plasmid DNA contamination
Posted 14 July 2013 - 11:29 PM
I have a problem with my transfections.
I recently isolated pDNA(plasmid DNA) for transfection using Qiagen Midi prep kit and used the elute for transfections.
But after seeding the cells (adhearent cells) and transfection, i find that the cells are contaminated.
I do not know what happened.Is it due to pDNA contamination, If yes what would be the best option to purify pDNA.
PLEASE HELP ME.
thanks in advance.
Posted 14 July 2013 - 11:35 PM
Posted 14 July 2013 - 11:39 PM
Edited by harry348, 14 July 2013 - 11:40 PM.
Posted 14 July 2013 - 11:41 PM
Posted 15 July 2013 - 01:17 AM
If it is fungus, it is most likely to have come from the incubator.
As it is plasmid DNA you are working with, it is probably easiest to do a fresh prep rather than worry about the source.
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