GST tag fusion protein--- help pleaseGST activity
Posted 14 July 2013 - 09:58 PM
I've been having a hard time to purify a GST tag protein. I use the pGEX4T-1 vector and so my protein is GST tagged.The fusion protein is partialy soluble.Then I purified my protein ,but there was no activity.Is there any one can help me ?
Posted 17 July 2013 - 03:50 AM
I`ve purified the fusion protein by the Glutathione Sepharose ，the sds-page results is right .Now I am tring to cleavage the GST tag by THROMBIN.Wish me good luck!Thank you again!
Posted 17 July 2013 - 04:29 AM
Posted 17 July 2013 - 05:31 PM
I have tried several times to purify the protein,and I it`s really a hard work .Could you give me some advise about using thrombin to cleavage the FUSION？