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purify protein from native gel


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#1 Kaire76

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Posted 13 July 2013 - 06:25 PM

Hi all,

Wanted to know if anyone has experience purifying proteins from native PAGE gels for downstream applications like EMSA? I was thinking about using invitrogen's NativePage Bis-Tris Gel system and cutting out my band of interest and eluting from gel. Is this nuts?


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#2 mdfenko

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Posted 15 July 2013 - 03:50 AM

not at all. we've used that procedure for some difficult to purify proteins. we prepare prep size and thickness gels to load large amounts of protein, cut and stain a lane and use that to line up the band of interest.

we used the resulting purified proteins to inoculate rabbits.
talent does what it can
genius does what it must
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