Smeared Bands after Ponceau Staining?
Posted 13 July 2013 - 07:56 AM
I have observed smeared looking bands on my Ponceau-stained PVDF after transfer. My samples are from bone, so I'm not sure what is the cause for this and/or if I should be concearned. Other than ~3-4 defined bands, the rest just looks smeared... (please see photo)?
I'm not sure if this is what protein degradation looks like, but I do add appropriate protease/ phosphatase inhibitors to my buffer?
Any ideas/ suggestions would be greatly appreciated!
Posted 15 July 2013 - 01:21 AM
Posted 15 July 2013 - 03:56 AM
do you boil the samples in the sds loading buffer? if so, try incubating at 60-70C for 10-20 minutes instead of boiling.
Edited by mdfenko, 15 July 2013 - 03:56 AM.
genius does what it must
i do what i get paid to do