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something very basic, why called "event-specific detection" - PCR, RT-PCR and Real-Time PCR - BioForum

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something very basic, why called "event-specific detection"


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#1 lactamase

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Posted 13 July 2013 - 07:39 AM

Hi all,

I am preparing some research for detecting Genetically modified food, and found that people usually use the word
"event-specific detection" for describing the RT-PCR detection of the 35S promoter and NOS terminator.

Why this name is used...? what exactly the meaning of "event-specific" mean ?

Thx and Cheers

#2 tkf

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Posted 15 July 2013 - 08:47 AM

event-specific detection is a PCR method to detect a transgene from one particular GMO. If the sample contains many GMOs, only the presence of that specific GMO will be answered by this method.

http://gmo-crl.jrc.e...Publication.pdf

This european commission document is for your reference (although a difficult read in my opinion) Posted Image See page 50

Edited by tkf, 15 July 2013 - 08:47 AM.





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