Resuspending primers calculation
Posted 11 July 2013 - 06:55 PM
"convert from nmole to umole then divide by the desired concentration in umole/L. The umoles cancel out giving the needed volume in liters. We then convert liters to ml."
Still my question is the initial concentration wich comes in nm is nm/L or nm/?? Because molarity is always expressed for a volume of solution. Then when we divide we are not dividing only nm but nm/some volume
Please help I'm an anthropologist so this part of work is hard for me. Sorry for my english!
Thank you in advance,
- ramankoson likes this
Posted 11 July 2013 - 08:37 PM
To put it in a simple way, if you have x nmol dry weight primer, you add 10 * x ul TE buffer, you will obtain 100 uM/L solution.
Posted 19 August 2013 - 03:48 PM
Thank you very much for your answers. My confusion is with nomenclature indeed.
Posted 20 August 2013 - 06:23 AM
We multiply nmol of primers by 50 to find the micro liter of water that should be added to them.
primer GAPDH-F nMol is 20
so multiply it by 50=> 20*50= 1000
now add 1000 microlitter of clean water to it and mix.
now 1micro litter is enough for 10 to 50 micro litter of pcr reaction.
Edited by memari, 20 August 2013 - 06:26 AM.