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Bought HEK293T cells from ATCC -- passage number question


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#1 lehatat

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Posted 11 July 2013 - 02:36 PM

A few months ago, someone accidentally threw away all of my frozen aliquots of HEK 293T cells (yes, it sucked). I use them to produce AAV and lentivirus. Anyway, I bought these from ATCC and they arrived today. The documentation says they are at passage number 17. Passage 17 is already a high passage number for me to attempt a transfection (I like it closer to passage no. 10). I've never purchased a cell line before; I have always gotten them from another lab (my old cells came from another lab with no more info than HEK293T passage number 7). It is normal to buys a cell line and have a higher passage number? Do I start with passage number 1 with purchased cell lines?

Thanks!

#2 bob1

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Posted 13 July 2013 - 07:41 PM

The parent line HEK293 has been around for a long long time and would most have been much higher than p17 when the T addition was made. The problem with passage number is that it is a bit lab dependent as to how it is measured, for instance many labs, when they get a line in, assign it as p0 - so it is likely that your p7 ones were actually much higher passage than 7.

There shouldn't be any problem with transfecting at much much higher passage numbers than you have been using.




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