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HPLC method for mouse creatinine measurements in a diabetes type 1 model


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#16 esbena

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Posted 07 August 2013 - 12:57 AM

This is what I interpolate from the standard curve:

 

~ 0.05 mg/dL

 

and this is a published average:

 

~ 0.205 mg/dL 



#17 esbena

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Posted 07 August 2013 - 02:55 AM

This is what I get when I interpolate from my standard curve (given above)

 

0.05 mg/dL

 

(but is this just the concentration in the injected 3 ul of the 25 ul in the vail? How do I relate this to the published average concentration in serum??

 

and this is the published average for the same strain of mice, and approx. same age:

 

0.205 mg/dL



#18 mdfenko

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Posted 08 August 2013 - 12:55 PM

concentration is the same no matter how much you inject. actual mass injected depends on concentration and volume of the injection.

 

the only thing i can think of for your differences (other than possibly abnormal mice) is interference by the baseline and/or neighbor peaks.


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