Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

What part of the promoter to clone

promoter molecular biolo

  • Please log in to reply
2 replies to this topic

#1 Johndg2

Johndg2

    member

  • Active Members
  • Pip
  • 7 posts
1
Neutral

Posted 10 July 2013 - 08:58 PM

My advisor is asking me to amplify the promoter of my gene of interest to be clone into a luciferase vector so we can test the expression under specific conditions. The problem is that I'm not sure how much or what to amplify. Does anyone have any suggestions? Thanks in advance!

#2 pcrman

pcrman

    Epigenetist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,160 posts
65
Excellent

Posted 10 July 2013 - 10:15 PM

It depends. If you want to have guaranteed promoter activity which recapitulates endogenous promoter, the longer the better. I would say 1-3 kb should be fine. You should also include some sequence (50 - 100 bp) downstream of the TSS.

#3 Johndg2

Johndg2

    member

  • Active Members
  • Pip
  • 7 posts
1
Neutral

Posted 11 July 2013 - 01:45 PM

Thanks for your help! I will keep what you suggested in mind.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.