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Fc block for flow cytometry

flow cytometry Fc block CD16 CD32 CD64

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#1 Tarique



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Posted 10 July 2013 - 04:16 PM

I am using human monocytes/macrophages. Before performing flow cytometry, I block cells with 2% FBS in PBS solution. Should I use commercial Fc blocker to get rid of nonspecific antibody binding? Secondly, I am interested to see CD16, CD32 and CD64 expression. In that cases, should I block my cells? Yes, I am using isotype control in my flow panel.

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