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How did they do this? Microinjection? Transfection?

transfection microinjection amyloid NT2 protein

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#1 NeuroADResearch



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Posted 09 July 2013 - 04:04 PM

Hi! I was reading

http://books.google.... stress&f=false

and was wondering how the researchers studied the amyloid-beta protein with their NT2 cell culture. Did they transfect the cells to produce the protein or did they microinject the protein itself?

Thanks for the responses!

#2 Tabaluga


    Making glass out of shards

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Posted 09 July 2013 - 05:55 PM

Is it possible for you to get access to the original article referenced at the bottom of the text ?

Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.


#3 Winegarden



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Posted 12 July 2013 - 08:29 AM

According to the text you linked to they used oxidative stress to induce amyloid-beta. NT2 cells express Abeta naturally and oxidative stress triggers the expression. Thus, they compared the base level of expression (without chitosan) to the level of Abeta expression in the presence of chitosan in order to determine chitosan's protective ability.

The actual paper is available here:


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