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Can I quantify restricted plasmid and oligos?


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#1 Indhumathi

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Posted 08 July 2013 - 11:19 PM

Hi
I just want to know whether it is a suggested practice to use UV spectrophotometer to quantify restriction digested plasmid and oligos?
Indhu

#2 Trof

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Posted 09 July 2013 - 12:41 AM

I don't see a reason why not. Our primer supplier sends an OD measurement along with the synthesis report.
If you have dsDNA of exact length you can even calculate a copy number from the concentration which is in case of plasmids often more important.

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

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#3 Indhu

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Posted 09 July 2013 - 10:29 PM

Thanks a lot. How do I calculate the copy number from the concentration?
Indhu

#4 Trof

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Posted 10 July 2013 - 02:14 AM

You can use the calculator I linked (or any other). You need to fill in the concentration and the bp length.

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

'Normal' is a dryer setting. - Elizabeth Moon





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