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Neutralization assay calculation

Assay

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#1 monroe

monroe

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Neutral

Posted 08 July 2013 - 11:18 AM

Hi All,
I am new to this assay and I had a very basic calculation question. In neutralization assay I incubate 50ul of 100ug/ml ab with 50ul virus for 1H at 37 and then add 100ul of cells. My question is what is the final concentration of the Ab that I am testing. Is it 50ug/ml (1+1 = Ab+Virus) or 25ug/ml (considering the dilution factor after adding cells (1+1+2: Ab+Virus:cells).

I appreciate your response.
Thanks.





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