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Using 96 well plates for flow cytometry - cells adhering

flow cytometry

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#1 Elioelio

Elioelio

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Posted 08 July 2013 - 02:59 AM

I'm carrying out Annexin V assays on live cells, and cell cycle analysis (using PI) on fixed cells. I'm using 96 well plates as I have so many samples. I was worried that, as the cells are adherent, then over the course of the assay (~2hrs) the cells would start to adhere to the plate. I staggered the samples and found there was not much difference between the first control and the last on the plate.

However, I'm worried that the adhering may make a difference that I'm not realising, as I'm not getting great results. Alot of my control cells are dead (~4-6%). Could this be that the live cells are adhering to the plate?

Does anyone have experience of using 96 well plates with adherent cells? I thought about buying some round bottom plates to see if that made a difference.

Any ideas appreciated.
Thanks





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