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Pseudomonas aeruginosa 14 with pUC19?

PA14 pUC19

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2 replies to this topic

#1 tihong10

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Posted 08 July 2013 - 12:48 AM

Hello,

I was wondering if anyone can tell me if a plasmid (pDsRed-Express2**) which has a pUC19 "backbone" be expressed in Pseudomonas aeruginosa 14?

The recommended propagation strain is E. coli DH5a but I wanted to electroporate the plasmid into PA14.
Can someone tell me if this is possible or if there are any articles that have done this?

Thank you,
Ted

**http://www.snapgene....DsRed-Express2/

#2 phage434

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Posted 08 July 2013 - 05:33 AM

It will probably work.

#3 GaryJing

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Posted 08 July 2013 - 06:30 AM

I copied the info below from the manual of pDsRed-Express2 vector, hope it's helpful for you.

pDsRed-Express2 is primarily intended to serve as a source of DsRed-Express2 cDNA. The flanking MCS
regions make it possible to excise the DsRed-Express2 coding sequence and insert it into other vector
systems. The vector can also be used to express DsRed-Express2 in bacteria.






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