monitoring gene silencing
Posted 30 March 2004 - 12:51 AM
i've decided to use RNAi as a method of functionally studying my gene of interest, and have a qeury you guys might be able to help me with.
in the absence of a fluorescent or confocal microscope, how can i confirm the gene silencing effect before looking at functional experiments? can i use basic RT-PCR or do i have to use some other method?
any help is greatly appreciated
Posted 30 March 2004 - 07:51 PM
Posted 30 March 2004 - 11:45 PM
Posted 04 April 2004 - 11:40 PM
Posted 14 April 2004 - 05:40 PM
In my case, my gene gives no visible phenotype and its knockout function give no phenotype either. thus my advise is to seek a phenotype to assess the functional importance of your gene.