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Use magnetic beads to purify restriction enzyme digestion product

DNA purification

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#1 tky



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Posted 07 July 2013 - 06:35 PM

Dear all,

I am asking whether it is possible to use magnetic beads (Ampure beads from beckman) to purify restriction enzyme digestion product instead of phenol chloroform isoamyl alcohol. It has been said the latter one can inactivate enzyme like CIAP.but I perfer a time saving purification method.

will the CIAP be take over to next step reaction if I use magnet beads for DNA purification.

#2 phage434



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Posted 08 July 2013 - 05:30 AM

CIAP is a horrible enzyme. My first step would be to design a protocol to eliminate its use. Switch to a temperature sensitive phosphatase, or eliminate the phosphatase entirely. Then, you can heat inactivate and then use beads to purify.

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