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TSAP in NEB buffer

restriction digestion cloning

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#1 tky

tky

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Posted 07 July 2013 - 06:20 PM

I have a question regarding the dephosphorylation of the vector fragment.
I am thinking to add TSAP (Promega) to NEB KpnI HF/NotI HF reaction (in CutSmart Buffer) since the working temperature are 37 degree for three enzymes.
But I am not sure whether TSAP is compatible with CutSmart buffer or not.

i hope you can share your experience on this.
Thank you very much in advance,





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