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Isolation of a Novel Antibiotic Resistance Plasmid DNA from Hospital Isolates of

plasmid

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#1 Dr. shakibaie mr

Dr. shakibaie mr

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Posted 05 July 2013 - 06:52 AM

Abstract

Emergence and dissemination of antibiotic resistance plasmids are major concern for hospital care system and

increases the cost and decreases effectiveness of available antibiotics used in treatment of hospitalized patients.

In this study two Pseudomonas aeruginosa, two Escherichia coli, and a Klebsiella pneumoniae were isolated from

Intensive Care Unit (ICU) of a university hospital, in Kerman, Iran. K. pneumoniae exhibited resistance to all antibiotics

routinely used in our hospital for treatment of patients except meropenem, while, the other isolates were sensitive to

carbapenems and ciprofloxacin. Plasmid analysis of the selected isolates showed the presence of a single plasmid

with molecular weight 65Kb in the P. aeruginosa isolate1. The plasmid was named as pKUM and belonged to

incompatibility group -2 (IncP-2). Conjugation by filter mating revealed that resistance associated with gentamicin,

kanamycin, cefotaxime, and ceftazidime phenotypes were transferred to E. coli ATCC25922 (Rifr) recipient cells at the

frequencies of 3.13 × 10-5 and 5.3 ×10-7 respectively. The results were further supported by curing and transformation

experiments. MIC to cefepime was ≥30 μg/mL both in donor as well as the transconjugants while decreased to 0.5μg/

mL in cured derivative. The plasmid pKUM was quite stable (86%) in both donor cells and the recipient. From above

results we concluded that resistances to third generation of cephalosporins, aminoglycosides and cefepime in P.

aeruginosa isolate1 were indeed encoded by a conjugative plasmid. Acquisition of cefepime resistance through

plasmid complicates the therapy of neutropenic patients in ICU and increases the cost and mortality of these patients.

published in

Jafar E, Shakibaie MR, Poormasoomi (2013) Isolation of a Novel

Antibiotic Resistance Plasmid DNA from Hospital Isolates of Pseudomonas

aeruginosa. J Clin Exp Pathol 3: 140. doi:10.4172/2161-0681.1000140

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Edited by Dr. shakibaie mr, 05 July 2013 - 06:56 AM.






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