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DNA laddering

apoptosis dna ladder apotarget

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#1 carollee

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Posted 04 July 2013 - 07:32 PM

Based on the kit ApoTarget by invitrogen, DNA fragmentation was seen to have a slight laddering effect (Image 1 below)

ApoTarget.jpg


The outcome I got is much of a smearing effect (Image 2 below)

Positive Control.jpg
where L1: 1kb ladder, L2: negative control, L3: positive control

Would the result I got be considered as DNA fragmentation even without the obvious laddering effect?

#2 Curtis

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Posted 05 July 2013 - 02:56 AM

Necrosis gives smear. Apoptosis gives fragmentation (the lowest band approximately 180-200 bp). Try loading your sample again and take a better picture. Half of your gel is missing. I have done many fragmentation assays, your sample is very faint.





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