Qiagen mini prep cell fragment pellet
Posted 03 July 2013 - 08:49 AM
I fund when using the Qiagen miniprep kit, after lysis and then neutralisation with N3, that the cell debris I get is impossible to pellet. It forms a weird semi-solid that floats between what seems to be two different phases. It really limits the amount of supernatant I can get.
I remember experiencing this before but it's particularly bad at the moment for me. Any advice much appreciated.
Posted 03 July 2013 - 12:23 PM
Posted 03 July 2013 - 03:19 PM
You can always filter with a coarse pore filter, such as cheese cloth.