human ELISA for bovine samples? (T4, cortisol, progesterone)
Posted 02 July 2013 - 09:33 PM
I want to test the levels of free T4, Cortisol and Progesterone in bovine serum samples. I was wondering if I could use available ELISA kits for the three molecules that are advertised for human serum samples. As the molecules in question are the same among both species, shouldn't it be possible to use the 'human' kit?
The only problem I could see here is, that the kits come with standards, that are probably in human serum and that could lead to problems in quantification.
Posted 04 July 2013 - 07:27 AM
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
I never trust anything that can't be doubted.
'Normal' is a dryer setting. - Elizabeth Moon
Posted 19 July 2013 - 11:27 AM
"Cross reactivity" in the moderator response is used incorrectly. The serum is not a cross reactant. Cross reactivity testing is done between the test kit ab and a structurally similar compound. (example: BhCG kit...testing with LH, TSH, FSH...amphetamine kit testing with D, L, DL amphetamine, methamphetamine, etc).