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Weird gel banding for apoptosis

apoptosis band gel electrophoresis

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3 replies to this topic

#1 carollee

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Posted 30 June 2013 - 06:42 AM

Hey guys,

I'm currently doing an apoptosis assay using the protocol from http://www.celldeath...th/dnaladd.html.

And when run on gel, the results I got had some smearing above the well. Anyone has encountered this before and know why did this happened?

CytoExtr Apoptosis (edited).jpg
L1: 1kb ladder
L2: Negative control (no apoptosis)
L3: Positive control (apoptosis present)

#2 memari

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Posted 30 June 2013 - 04:21 PM

Your DNA has stopped in wells because Proteinase K has not done its job completely.
-----
Babak Memari

#3 carollee

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Posted 30 June 2013 - 07:18 PM

Thanks.

Do you have any idea about the present of smearing above the well too?

#4 almost a doctor

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Posted 01 July 2013 - 01:13 AM

I don't think that what you see above the well is smearing, but just the Ethidum bromide running in the opposite direction to the DNA. (Assuming you are using EtBr).





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