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Percent helicity of acylated peptide greater than 100%

Circular Dichroism Peptide

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#1 Ben Tambling

Ben Tambling


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Posted 28 June 2013 - 08:18 PM

I used Circular dichroism spectroscopy to determine the helicity of my acylated peptide. I used the formula [theta] = 100 theta/lcn to convert the observed milidegrees ellipticity to mean residue ellipticity (deg cm^2/dmol). Then I used the formula: alpha-helix=-100(theta222+3000)/33000 to find the helicity of the lipopeptide. I got a value greater than 100. Is this correct? Is there a correction for this?

Any suggestion will be greatly appreciated.

Thank you! :)

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