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Transfecting three plasmids into hES cells

transfection stem cells

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2 replies to this topic

#1 kaveh

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Posted 28 June 2013 - 03:52 PM

Hi,

I would like to transiently transfect 3 different plasmid DNAs into human embryonic stem cells by lipofectamine. If cytotoxicity is not a huge issue, what amount of each plasmid would you use in a well of 6-well plate.

any suggestion is appreciated.

k

#2 pcrman

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Posted 01 July 2013 - 11:50 PM

I think what matters is the total amount of plasmids transfected. You can use a high dose that does not cause huge cytotoxicity.

#3 GaryJing

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Posted 05 July 2013 - 06:47 PM

2 ug for each plasmid, and 6 ug in total





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