Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Lentivirus contamination

  • Please log in to reply
1 reply to this topic

#1 CAMPE983



  • Members
  • Pip
  • 1 posts

Posted 28 June 2013 - 06:37 AM

Hi all, yesterday I was filtering a supernatant containing lentiviral particles (third generation, VSVg as coating protein), something went wrong and some drops squirted out of the hood. I found some drops on my bare skin and some of them on my clothes under the labcoat. Of course I immediately washed my arms with water and ethanol. My question is: is there any chance that lentiviruses can penetrate the skin layer? Also, how long do you think they can leave if the liquid dries? (eg on surfaces or on my tshirt!). I read that HIV viruses can live for some minutes in the air, can I assume the same for lentiviruses? I am pretty sure I did not touch my eyes/mouth but I am also scared about aerosols...can they come out of a cell culture hood?
The transgene was a shRNA against a mouse gene, so I don't think it should be dangerous....but I am worried about insertions in my genome....
Thank you so much for your answers....I am kind of scared right now....

#2 gfischer



  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 197 posts

Posted 28 June 2013 - 10:23 AM

Lentiviruses cannot cross the skin, you will be fine.
Above all things, if kindness is your king,
then heaven will be yours, before you meet your end

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.