real time sybergreen concentration
Posted 25 March 2004 - 04:33 AM
any body known realtime sybergreen concentration in pcr reaction.
i planed external addition all sybergreen kit (roche) kontent???
Posted 04 April 2004 - 04:45 PM
other than that, maybe look up these kits anyway - they might have what concentrations they use in them. or contact the companies and ask them (although it might be proprietry information).
Posted 05 April 2004 - 12:29 PM
Posted 01 August 2004 - 11:50 PM
Maybe these two papers can help you (I found them recently):
- "A quantitative fluorescence-based microplate assay for the determination of double-stranded DNA using SYBR Green I and a standard ultraviolet transilluminator gel imaging system.", Vitzthum F, Geiger G, Bisswanger H, Brunner H, Bernhagen J., Anal Biochem. 1999 Dec 1;276(1):59-64
- "Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications.", Zipper H, Brunner H, Bernhagen J, Vitzthum F., Nucleic Acids Res. 2004 Jul 12;32(12):e103.
Posted 03 August 2004 - 03:19 PM