Co-IP vs Pull down
Posted 27 June 2013 - 11:35 PM
I'm new to both Co-IP and GST-pull down. I was reading a paper where the author confirmed interaction between two proteins A and B uisng Co-IP. However he also did GST and his-tag pull down of both proteins? I thought doing one was sufficient? what is the difference between the two?
Posted 28 June 2013 - 03:18 PM
They will have used both as a confirmatory approach for the interaction, similar to using both co-IP and confocal to demonstrate that proteins interact.
Posted 02 July 2013 - 07:31 PM