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Selection of E.coli competent cells


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#1 xxcici

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Posted 27 June 2013 - 02:31 PM

I am going to get a pT7 Blue vector with cDNA of interest cloned. After I receive the plasmid, I want to fo transformation to get more plasmids. Which commercial E.coli competent cells can I use for plasmid propagation? What about One shot Top 10?

#2 bob1

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Posted 27 June 2013 - 03:20 PM

Yes, those would work, or any other commercially available variety. If you have a strain of E.coli in your lab that you would like to use, it is very easy to make your own competent cells - protocol here

#3 xxcici

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Posted 04 July 2013 - 11:26 AM

Thank you, Bob.




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