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Chelating Aluminum from cell culture


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5 replies to this topic

#1 Mano

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Posted 27 June 2013 - 08:41 AM

Hi, I wanna chelate aluminum from cells in cell culture after their treatment with AlOH3 for some time, any idea how can i do it.Thanks Posted Image

Edited by Mano, 27 June 2013 - 08:41 AM.


#2 bob1

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Posted 27 June 2013 - 03:25 PM

Without damaging the cells? If you don't mind the damage you could use a metal ion chelator such as EDTA or EGTA.

#3 mdfenko

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Posted 28 June 2013 - 04:36 AM

if you can get your hands on some, you can try feralex. it's being used to chelate aluminum and iron in vivo.

see these papers:

about feralex

synthesis

one of my coworkers has a different, more efficient, synthesis method and others here have used it in their research (unfortunately, i'm not privy to all of the information or i could tell you more)
talent does what it can
genius does what it must
i do what i get paid to do

#4 Mano

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Posted 30 June 2013 - 08:44 PM

Thanks alot :)

#5 mdfenko

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Posted 01 July 2013 - 10:18 AM

i spoke with my coworker and he is willing to give you a sample of feralex. do you want me to pm you his email or do you want to pm me yours so that the two of you can get in contact?
talent does what it can
genius does what it must
i do what i get paid to do

#6 Mano

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Posted 02 July 2013 - 10:17 AM

oh, Wow Thanks alot for your help, i sent u a message




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