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chemotherapeutic-resistant cell lines

how can I produce?

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1 reply to this topic

#1 moljul

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Posted 27 June 2013 - 03:54 AM

hello there,

does anyone of you know how to produce chemotherapeutic-resistant cell lines?

thanks in advance

#2 bob1

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Posted 27 June 2013 - 03:28 PM

A basic procedure would be to take cells, treat with the drug at a non-lethal dose (say LD50) and select cells that are resistant. Another way would be to add a resistance gene by stable transfection.




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