I'm trying to decide which cell line to use for Alzheimer's disease (I am transfecting through electroporation APPswe).
I want to investigate the effects of a chemical on Aß42, tau proteins, and reactive oxygen species. I know that APPswe does show both Aß42 and tau phosphorylation, and ROS. I am not sure if I should use a microglial cell culture to study the effects of the chemical on ROS, because the ROS are mainly studied with microglial cells.
What have you heard or know is the best cell line for this cell culture for AD, HEK293 or SH-SY5Y?

Also, what protocol would you recommend for the medium of that cell line? Serum-free/with serum?
Thanks! Any further advice would help!