Posted 26 June 2013 - 01:55 PM
Posted 27 June 2013 - 03:31 PM
Posted 28 June 2013 - 07:04 AM
I did a side by side comparison of lysate samples to which I added Rnase inhibitor and samples where I didn't, and there wasn't any difference in signal between them.
I've harvested fresh cells and resuspended them so that they're about twice as concentrated as before, I'll see if that makes a difference or not.
Posted 02 July 2013 - 08:32 AM
I think the next thing I'm going to try is playing with the concentration of my labeled and unlabeled UTP. I did this when opitimizing the T7, but for cell lysate the optimal concentrations may be different.