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Double strand cdna synthysis

Molecular Biology cDNA

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4 replies to this topic

#1 zfatima

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Posted 26 June 2013 - 10:24 AM

- im trying to synthesize double strand cDNA (viral) using invitrogen's superscript double strand cDNA synthesis kit but having problems which surely are related to rna concentrations. has anyone used this kit and at what rna concentrations they proceeded with the synthesis? Kindly help!

#2 Curtis

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Excellent

Posted 27 June 2013 - 04:31 AM

I have not used it, but I can tell you gsp primers synthesize better than random hexamers.

#3 zfatima

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Posted 27 June 2013 - 11:37 AM

gene specific? yep have been using them to synthesize first strand cdna.

#4 Curtis

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Excellent

Posted 28 June 2013 - 09:17 AM

1ug rna is enough

#5 zfatima

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Posted 28 June 2013 - 02:07 PM

- okay thank you.





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