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Calculate sample's concentration after reconstitution

calculation HPLC reconstitution delution sample

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#1 ayyash



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Posted 26 June 2013 - 04:56 AM


i'm had a sample of 1L that gone through extraction & evaporation. i used 0.5ml of methanol for reconstitution before injecting the sample to HPLC. i made a calibration curve using a standard solution but now i'm confused about how to display the concentration i got from the sample. for ex. i got 0.2ug/ml how i'm gonna explane this is for the 1L ?
please help .. i'll really appreciate it


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