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Selection of needle size to homogenize cells for RNA extraction


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#1 wdyeo

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Posted 26 June 2013 - 02:59 AM

Dear All,
I used RNeasy Mini Qiagen to extract RNA from cell culture.
But if i use 26G needle instead of 20G needle (as written down in the handbook) to homogenize the cells in cell culture, will it affects the quality of RNA or i loss more RNA etc?
Help..


Edited to make font readable. Bob

#2 Curtis

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Posted 27 June 2013 - 04:45 AM

I have used that red color kit, but never used any needle.

#3 wdyeo

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Posted 28 June 2013 - 02:45 AM

If you do not use syringe and needle, how do you homogenize your cells?
Thanks ~

#4 Curtis

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Posted 28 June 2013 - 09:19 AM

you said cell culture. they don't need homogenization, unless you mean tissue cells?




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