Selection of needle size to homogenize cells for RNA extraction
Posted 26 June 2013 - 02:59 AM
I used RNeasy Mini Qiagen to extract RNA from cell culture.
But if i use 26G needle instead of 20G needle (as written down in the handbook) to homogenize the cells in cell culture, will it affects the quality of RNA or i loss more RNA etc?
Edited to make font readable. Bob
Posted 27 June 2013 - 04:45 AM
Posted 28 June 2013 - 02:45 AM
Posted 28 June 2013 - 09:19 AM