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How do tandem integrations of transgene typically line up?

tandem integration transgene knockout

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#1 assembler01

assembler01

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Posted 25 June 2013 - 12:00 PM

When you see multiple tandem integrations of a transgene (in this case electroporated and designed with 5' & 3' homologous arms for recombination ideally), how do they usually line up? Do they line up perfectly head to tail, such that you see the last 3' base of the first transgene and then the first 5' base of the next? Do they randomly insert such that pieces of each 3' or 5' end are missing or there is genomic DNA in between? I know they can sometimes insert in other locations in the genome as well but I was just wondering about tandem integrations. Would they ever invert and some be head to head or tail to tail?

Thanks.





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