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ammonium sulfate precipitation


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#1 Raj_9

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Posted 24 March 2004 - 02:13 PM

Can anybody tell me whether I can do ammonium sulfate precipitation after homogenizing my tissue with Triton x-100.

Or

Do I have to dialyse first to remove Triton x-100?

I am trying to purify an enzyme.

Thanks.

#2 phdconsult

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Posted 01 April 2004 - 06:46 PM

Triton is rarely removable by dialysis - sticks to the membrane. We recommend diluting the protein sample 1:2 so the effective Triton concentration goes down and then doing the ammonium sulfate precipitation. Do not use powder ammonium sulfate, use a saturated solution of the salt and cool everything down to 4C. Good Luck

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