Edited by flyzapper, 24 June 2013 - 12:54 PM.
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Mitochondrial ribosomal control gene for qPCR?
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Posted 24 June 2013 - 12:54 PM
I'm designing a qPCR experiment in Drosophila, and I need a good control gene for some genes that are expressed at low levels. I want to use a control gene that is expressed at a similar level so I can get the best expression data for my genes of interest. One of my best candidates is a mitochondrial ribosomal gene, mRpL50. Has anybody used a mRpL gene as a control before? Any other suggestions for good low-level control genes?