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Electroporate lentiviral vector for stable cell line


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#1 Mindologist

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Posted 23 June 2013 - 07:15 AM

Hi all scientist here,
I'm just curious whether I could get stable cell line if I just electroporate lentiviral vector. This is because I just feel bothered to go through packaging step.
I'm totally new at viral vector.
So, please give any comments, advice, or lecture.
Thanks in advance.
Have a good day.
^^v

#2 pcrman

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Posted 23 June 2013 - 07:45 AM

Yes, you can. Without packaging, it is just a plasmid. The thing is that you may not have the same efficiency as viral infection.

#3 GaryJing

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Posted 02 July 2013 - 10:49 PM

Just take it as a normal vector if you wanna skip the step of packaging lentivirus




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